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apex 2x taq master mix

apex 2x taq master mix

Description

Our product 2X Taq PCR Master Mix is ​​a ready-to-use 2X premix solution that includes Taq DNA polymerase, dNTPs, a pre-optimized buffer system and dye. Taq DNA polymerase synthesizes DNA under appropriate conditions from single-stranded templates in the presence of gene-specific primers and dNTPs. It is a recombinant product extracted from E. coli in which the Thermu aquaticus DNA polymerase gene is expressed.

Taq DNA polymerase has a 5’→3 DNA polymerase and a weaker 5’→3′ exonuclease ability, however, the 3’→5′ exonuclease has no ability, meaning that a DA overhang appears at the 3′ end. Will give It can be used in TA cloning using this attribute. In the PCR reaction, the elongation rate of Taq DNA polymerase is approximately 1–2 kb/min, depending on the complexity of the gene. For most templates, using 1kb/min.

The reaction system is ready to use by adding primer, template and ddH 2 O, simplifies experimental steps, reduces individual error and improves repeatability of results.

This master mix contains dye which means it can be electrophoresed directly after amplification without the need to add loading buffer. If the PCR product needs to be subjected to agarose gel electrophoresis, our product SYBR Safe DNA Gel Stain (Cat: A8743) is available.

Apex Bioresearch Products’ 2.0x Taq Master Mix (available from Genesee Scientific) contains everything you need for routine PCR except templates and primers. Given the sensitivity of PCR, and its tendency to increase even minute levels of DNA contamination, anything that serves to reduce the number of pipetting steps and the number of stock solutions is an advantage. Since many vendors now carry different types of master mix, choosing one over the other reduces the price and how well the master mix performs in your hands. And, since switching from a tried-and-true master mix isn’t something most of us are willing to do without good reason, it helps to have a free sample to try. I recently took advantage of a free sample offer from Genesys Scientific, the exclusive retailer of Apex Bioresearch Products.

apex 2x taq master mix
apex 2x taq master mix

 

All 3 amplification reactions contained 30 ng of template (pComb3X phagemid containing a human Fab insert), 0.2 µM each of forward and reverse primers, 0.2 mM dNTPs, and 0.625 units of polymerase. These reagent concentrations were obtained by diluting Apex™ Master Mix to 1X. The other 2 reactions were manually pipetted from the stock solution. After an initial denaturation step of 94 °C for 2 min, amplification was performed for 30 cycles of 94 °C for 15 sec, 56 °C for 30 sec and 72 °C for 2 min. Three microliters of a total reaction volume of 25 μl was subjected to agarose gel electrophoresis on a 1% TAE gel containing 1X GelStar nucleic acid stain (Lonza Inc.). A picture of the prison is shown below.

 

Properties

Ideal product for general PCR or other experiments
Quick and easy-to-use ready-made PCR mix
low cost per unit
that already contains dye

Storage
Store 2Xmaster Mix at -20°C.

Key Features

Taq DNA Polymerase 2x Master Mix Red Amplicon is an easy-to-use 2x reaction mixture with Taq DNA polymerase, NH4+ buffer system, dNTPs and magnesium chloride present. Each reaction requires 25 µl of 2x Master Mix Red. Add primer, template and water in a total reaction volume of 50 µl to successfully carry out primer extension and other molecular biology applications.
Taq DNA Polymerase 2x Master Mix Red offers several advantages. The set up time is significantly reduced. The possibility of contaminating the component stock is eliminated. The reduction in reagent handling steps results in better reproducibility. Standard tests can be set up with the confidence that the results will be consistent every time. There is no need to buy and use a separate loading die. Simply load a portion of the reaction product onto an agarose gel for electrophoresis and subsequent visualization. The red dye front runs at 1000 – 2000 bp on a 0.5 – 1.5% agarose gel.

 

 

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