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Atpase Assay buffer

Atpase Assay buffer

Description

ATPase (Adenosine Triphosphatase: EC 3.6.1.3) is an important enzyme for maintaining cell membrane potential, transporting ions and regulating cellular volume. It catalyzes the decomposition of ATP into ADP and a free phosphate ion. Hydrolysis of ATP is highly exergonic releasing energy that is used in many cellular processes. There are several classes of ATPases, including Na+/K+ -ATPase, H+/K+ -ATPase, Ca2+ -ATPase, etc. Mitochondrial ATPase deficiency is severe: for example, Na+/K+-ATPase deficiency increases anxiety-like behavior, whereas Ca2+-ATPase deficiency leads to exertional muscle pain syndrome. Therefore, accurate detection of ATPase activity is important for the diagnosis and mechanistic study of some of these diseases. BioVision’s ATPase Activity Assay Kit provides a quick and easy way to monitor ATPase activity in a variety of samples. In the assay, ATPase hydrolyzes free ATP and a free phosphate ion to ADP, and through linked reactions, a strong, stable chromophore is generated (OD 650 nM). The assay is simple, sensitive, high-throughput adaptable and produces 0.005 U/L. Can detect ATPase activity less than

ATPase Activity Assay Kit
ATPase Activity Assay Kit

Cat # +SizeK417-100Size100 Assays Kit Summary • Detection Method – Colorimetric (OD 650 nm)
• Species Reactivity- Mammalian
• Application – The assay is designed to measure ATPase activity in biological samples with detection sensitivity ~5 mU/l Detection method Colorimetric (OD 650 nm) Species reactivity Mammalian application ATPase activity in biological samples or recombinant ATPase preparations Quick evaluation of Features and Benefits • Simple process; takes less than 1 hour
• Fast and Convenient Kit Component • ATPase Assay Buffer
• ATPase substrate
• ATPase Developer
• Phosphate Standard (10 mM)
• ATPase Positive ControlStorage ConditionsMultiple TemperatureShipping ConditionsGel PackUse For Research Use Only! Not for use in humans.

ATP Colorimetric Fluorometric Assay Kit from BioVision

ATP is the primary energy currency of living systems. Virtually all energy-requiring processes use the chemical energy stored in the phosphate bonds of ATP. ATP is made exclusively in mitochondria and a variety of genetic diseases can affect ATP formation in mitochondria. There are several commercially available ATP assays that detect femtomoles or less than ATP by measuring luminescence (eg Biovision Kit 254-200), but these kits require specialized luminescence instrumentation and use luciferase which It can be difficult to maintain in an active form. The BioVision newly developed ATP colorimetric and fluorometric assay kit is designed to be a robust, simple method that utilizes the phosphorylation of glycerol to generate a product that can be easily characterized by colorimetric (λmax = 570 nm) or fluorometric (Ex. /Em = 535/587 nm) methods. The assay can detect as little as 50 picomoles (1 µM) of ATP in various samples. The kit provides enough reagent for 100 assays

ATPase Activity Assay Kit (Colorimetric)
ATPase Activity Assay Kit (Colorimetric)

Introduction:

Adenosine-5′-triphosphate (ATP) is a central molecule in the chemistry of all living things and is used to monitor many biological processes. An accurate, reliable method for detecting minute ATP levels such as the luciferase/luciferin system has wide application. Conventional luciferase/luciferin ATP detection systems are unstable because luciferase rapidly loses activity. At Biovision, we have developed a highly stable luciferase; A genetically modified variant derived from luciferase of Diaphanes pectinalis (Chinese firefly), endemic to Yunnan Province, China. We designate our recombinant highly stable luciferase rLucHS. Compared to the normal phenotype of Photinus pyralis, rLucHS offers increased stability, excellent sensitivity and a wider and more physiologically relevant effective pH range. Below all pHs of ~8.2, rLucHS has a significantly higher relative activity than photoinase luciferase and is stable for weeks at room temperature and 60 min at 37 °C. Using the protocol outlined here, the magnitude range is approximately between 1 nmol to 10 fmol/assay. The specific activity of rLucHS is ~5 x 1011 RLU/mg protein. The assay can be fully automated for high throughput (1 sec/sample) and is extremely sensitive and ideal for the detection of ATP production or consumption in various processes and enzymatic reactions.

 

 

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