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BioMag Plus Concanavalin A

Description
Bead- and column-based separation methods rely heavily on the speed and ease of affinity binding systems. streptavidin, antibodies and
Lectin-like ligands are used to capture specifically tagged targets and for the isolation of cells and biomolecules that naturally express ligand binding partners.
The unique saccharide-binding properties of plant lectins, such as concanavalin A (Con A), have made them useful for the labeling and isolation of glycanpresenting cells and glycoproteins in serum and cell lysate. to affect lymphocyte activation, and
Lectins have additionally been used in cell adhesion studies to explore carbohydrate-based therapeutics.
Our Con A-Coated Biomag Plus Microparticles
They provide a convenient means to isolate manosyl- and glucosyl-containing glycoproteins and polysaccharides from serum or cell lysate, or to investigate other lectin/glycan-mediated processes. Use of BioMag Concanavalin A
CUT&RUN, a chromatin profiling protocol with several major advantages over chip. (see references)
Symptoms
Concanavalin A (Con A)
Covalently attached to functionalized BioMag®Plus particles for use in glycoprotein isolation from serum and cellular extracts.
Con A is a 104,000 Da protein consisting of four identical subunits, and
Exists as an active dimer or tetramer depending on pH. its carbohydrate binding
The partners are α-D-glucose and α-D-mannose,
With unmodified OH groups at C-3, C-4, and C-6, and terminal glucose residues of proteins and peptides.
Con A red blood cells (RBC)
Enhances, interacts with immunoglobulin glycopeptides, and is a lymphocyte mitogen. It binds to certain bacteria.
Con A bond is mediated by metal ions, which is stabilization. each
The binding site requires calcium and manganese ions, and EDTA
Or use of the buffer with other metal chelators will result in loss of carbohydrate binding capacity.
Mean Diameter: ~1.0μm
Concentration: 5 mg/ml
Con same: set by A280
Material
material supply
Contain a coated particle with 0.1% sodium azide in 3 mL or 10 mL of 10 mM PBS.
material required
1.5
mL or 2 mL microcentrifuge tube Mammalian Serum: PBS/Test
0.4 mL of 1:20 dilution in Binding Buffer: 1x PBS + 0.1% NaN3
+ 1mM MgCl2
+ 1mM MnCl2
+ 1m CaCl2
(pH 7.4)
Wash Buffer: 1x PBS + 0.1% NaN3
+ 1mM MgCl2
+ 1mM MnCl2
+ 1mM CaCl2
(pH 7.4) + 0.1% Tween® 20
Con A particle elution buffer: 5 mM Tris (pH 8.0) + 0.15 M NaCl + 0.05% SDS + 1 M glucose
Precision pipette 20-200μL, with disposable tips for dispensing 200-1000μL
Microcentrifuge Tube Separator: 1.5mL Magnetic Separator (Catalog Code LS001) Biomag Multi-6 Microcentrifuge Tube Separator (Catalog Code MS002)
Vortex mixer and tube rotator.

Process
Researchers are advised to optimize the use of particles in any application.
1. Prepare a 0.4 mL serum sample by diluting it 1:20 with 10 mM PBS.
2. Transfer 1mL of the BioMag Plus Con A particles to a clean microcentrifuge tube. Place the tube on a magnet to separate the particles from the solution.
Carefully remove and discard the solution.
3. Wash the particles by adding 1mL of binding buffer. Mix well.
4. Repeat the particle wash one more time. After the final wash, remove the supernatant.

5. Add 0.1mL binding buffer to the serum sample from step 1. Add the sample to the particles and mix well by inversion or vortexing to re-suspend the particles.
6. Place the sample on the tube rotator and mix for 10-30 min at room temperature.
7. Remove the sample from the rotator and place it in the magnetic separator. Carefully remove the clean supernatant.
8. Wash the particles by adding 0.5mL of wash buffer. Mix well by inversion or vortex mixing.
9. Repeat steps 7-8. Re-suspend the particles with 0.5mL wash buffer and place on the tube rotator for 5 min.
10. Repeat steps 7-9.
11. Replace the particles tube on the magnetic separator and carefully remove/discard the supernatant.
12. Add 250µL elution buffer to the particles. Shake the tube to re-suspend the particles and place the tube on the rotator for 10–30 min at room temperature.
13. Replace the particles tube on the magnetic separator and carefully remove the eluate and transfer to a clean microcentrifuge tube for later use or storage.
14. Repeat steps 12-13. Eluates can be pooled and precipitated. Store eluates on ice for immediate use or freeze for long-term storage.

BioMag Plus
BioMag Plus

NOTES
• Avoid the use of reagents with EDTA or other metal chelators, as this will reduce the effectiveness of the Binding Buffer.
• Protease Inhibitors may be used when sensitive glycoproteins are isolated.
• Low glycoprotein recovery may be attached by either increasing the elution incubation time beyond 10 minutes, and / or by boiling particles in 200µL of
SDS-PAGE sample buffer for 5 minutes and then magnetically separating the particles from the eluate. (Note: Boiling may detach some lectins and may
also release nonspecifically bound proteins.)
• Run eluate samples on an SDS-PAGE 4-20% Tris-Glycine electrophoresis gel and stain the glycoprotein bands using the GlycoGel Stain Kit
(Polysciences’ Catalog Code 24693) to visualize.
• After GlycoGel staining, stain the gel using Coomassie G250 (1mL or 2mL of 0.5% Coomassie G250 in 50% methanol and 10% acetic acid) to visualize
other protein bands.
• The removal of albumin and IgG from serum samples may improve the isolation of low concentration glycoproteins. If desired, use the BioMag ProMax
Albumin Removal Kit (Catalog Code BP658) and / or the BioMag ProMax Serum IgG Removal Kit (Catalog Code BP659).

 

Catalog Number Description Concentration (mg/mL) Volume (mL) Product Data Sheet Safety Data Sheet
BM551 BioMag Streptavidin 5 5, 50 PDS 551.pdf SDS MB232.pdf
BP628 BioMag Plus Streptavidin 5 10 PDS 621.pdf SDS MB232.pdf
BM568 BioMag Streptavidin Nuclease-free 1 10, 25 PDS 530.pdf SDS MB232.pdf
BP621 BioMag Plus Streptavidin/Biotin Binding Kit 5 5 PDS 621.pdf SDS MB232.pdf
SDS MB255 Wash/Coupling Buffer.pdf

 

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Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule

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Concanavalin A (Con A) Antibody

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  • 100 ug
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Concanavalin A ELISA Kit

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Concanavalin A, CF350 conjugate

29015 5x1mg
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Concanavalin A, CF640R conjugate

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Concanavalin A, CF680 conjugate

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Concanavalin A, CF770 conjugate

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Concanavalin A, CF405M conjugate

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Concanavalin A, CF405S conjugate

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Goat Concanavalin A ELISA kit

E06C0795-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Concanavalin A ELISA kit

E06C0795-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Concanavalin A ELISA kit

E06C0795-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Concanavalin A ELISA kit

E02C0795-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Concanavalin A ELISA kit

E02C0795-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Concanavalin A ELISA kit

E02C0795-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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