Nanocomposite double-network hydrogels (NCDN hydrogels) have recently been introduced to address the limitations of conventional DN hydrogels, such as the lack of diversity in network structure and restricted functionality. However, two challenges remain, which include time-consuming preparation and lack of shear-thinning and self-healing properties. Here, our approach for versatile growth of ncDN hydrogels is through the use of several interfacial crosslinking chemistries (i.e., noncovalent interactions with electrostatic interactions).
General Details
Agarose is obtained from seaweed and consists of repeated agarobiose units. During agarose gel formation, polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.
Application
Agarose LE is especially suited for analyzing fragments between 0.2 and 15 kb in: Analysis of PCR products
Examination of restriction endonucleases
Digests of plasmid, cosmid, and λ phage DNA
Electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques. Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.
Quality
Absence of DNase: none detected according to the current Quality Control procedures. Absence of RNase: none detected according to the current Quality Control procedures.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Agarose LE
PROPERTIES
form
powder
packaging
pkg of 100 g (11685660001) pkg of 500 g (11685678001)
