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Murashige & Skoog Modified Basal Salt Mixture

General Details
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients including high levels of nitrates and organic additives such as agar, sugars, vitamins, and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and kinetin (cytokinin/cell division promoter).
Application
Murashige and Skog medium have been used in plant tissue culture medium for the study of plant transformation.
Formula Version
With macro- and micronutrients and vitamins as described by Murashige and Skog (1962).

media formulation

Quantity
Formulated to hold 4.4 g of powder per liter of medium.
preparation note
Murashige and Skog medium can be made from a powder or by combining products that are major components of the complete M&S medium, such as macronutrient blends and vitamin blends. Murashige & Skog Medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxin (IAA) and cytokinin (Kinetin) to prepare a complete medium for growth plant tissue culture.

murashige-skoog-without-nitrogen
murashige-skoog-without-nitrogen
Solubility Water
Physical Form Solid
Storage Temp. 2-6 ┬░C
Grams of powder to prepare 1 Liter 0.78
Other Notes Plant Tissue Culture Tested
UPC / SKU M531

PRODUCT DESCRIPTION

Contains micronutrients and macronutrients as described by Murashige and Skog (1962), but without ammonium nitrate and potassium nitrate.

Sucrose, cytokines, vitamins and auxins should be added as needed.
Provided as 1 unit to make 10L, 25L, or 50L medium
Plant tissue culture tested
High capacity with low air consumption
SEPURAN® N2 A Hollow Fiber Membrane Gas
separator which is used
Compressed air is used to remove oxygen, resulting in N2 purity of 95-
between 99.5%. polymers especially nitrogen
tailored for production. Unlike other gas separation membranes,
SEPURAN N2 to membrane
Featuring very high flow capacity with very good air factor,
resulting in compression and
Operating cost is optimized.

murashige-skoog-without-nitrates
murashige-skoog-without-nitrates

Product Structure
The residual oxygen content was determined by measurement.
The “Production of N2” value is the inert gas content.
pressure is the retentate pressure, ie N 2
Stream discharge pressure produced.
inlet air air factor divided by “N2 produced” or
Gives the Air/N2 ratio. The lower the air factor,
The investment and operating cost will be the same.

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Evonik’s current knowledge and experience. However, Evonik
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Do not refer to trade names used by other companies
So the product concerned is neither a recommendation nor an endorsement, and does not imply that
The same products cannot be used.

 

 

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