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Direct PCR

Phire and Phusion Kits

What is Direct PCR?

Direct PCR (dPCR) is a method of direct DNA amplification from an animal or plant tissue sample without performing DNA isolation and purification steps. This technique greatly reduces experimental time and cost in genotyping and high volume projects. It also provides a better option when faced with the challenges of growing a sample in very small quantities where the purification step could potentially lead to sample loss.

In many ways, dPCR works like conventional PCR. Both techniques rely on template DNA, primers and a master mix or PCR reagents, and both use a thermal cycler for amplification. The primary difference between dPCR and conventional PCR is the tailored buffers used in dPCR. Modified elements of dPCR enable robust amplification despite the presence of PCR inhibitors often found in raw samples.

ALS SARS-CoV-2 RT-PCR 4G is entirely developed and manufactured at ALS in Portugal and is primarily designed for export. This new kit is able to detect all known SARS-CoV-2 variants, including British, Brazilian and South African variants, without cross-reacting with other known related viruses or common respiratory pathogens. The choice of genomic regions to be used as targets and the sequences of primers and hydrolysis probes were based on WHO, CDC and DGS recommendations.

ALS SARS-CoV-2 RT-PCR 4G Kit is an in vitro diagnostic medical device (CE-IVD) that uses real-time PCR nucleic acid amplification technology for the detection of SARS-CoV-2 in clinical samples from upper respiratory tracts. Uses up. The path of persons who may or may not be suspected of having COVID-19.

The kit uses RT-PCR technology, which consists of amplification of certain regions of the viral genome with its detection by specific hydrolysis probes. The SARS-CoV-2 genome target regions are highly conserved and correspond to two specific genes: the ORF polyprotein (ORF1ab gene) and the nucleocapsid phosphoprotein (N gene). The kit can also detect the gene that codifies the structural protein of the envelope (gene E) specific to the sarbecovirus subgenus.

Direct PCR Reagent
Direct PCR Reagent

Benefit

Rapid amplification: The amplification rate of polymerase is 6kb/min. A 1kb piece can be extended within 25min.
Long fragment amplification: For plasmids, DNA and other easy templates, the polymerase can effectively amplify >20kb. For the genome, the polymerase can effectively amplify >8kb. And for cDNA, the polymerase can effectively amplify >8kb.
High Specificity: With hot start technology, the polymerase is 100% inactive below 50 °C, and can only be restored by heating at 95 °C for 5 min.
High tolerance to impurities: can be directly amplified in whole blood, serum, cultured cells, and urine samples without prior DNA purification.
unit definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid-insoluble substance within 30 min at 74 °C.

 

Description Code Specification Category Technology
ALS SARS-CoV-2 RT-PCR Test for surfaces ALS-B01-Adv RUO Food /Water Virology Detection Real-Time PCR
ALS SARS-CoV-2 RT-PCR Test for Wastewater ALS-B02-Adv RUO Food /Water Virology Detection Real-Time PCR

 

Plant Direct PCR Kit

PD105-02 200 rxn (50 μl/rxn)
EUR 354

Direct PCR Complete Kit

S700 100 rcs
EUR 133

Direct PCR Complete Kit

S700-2 5x100 rcs
EUR 524

amfiSure Direct PCR Lysis Buffer

D0300-010 100ml
EUR 138

Blood Direct PCR Kit V2

PD103-01 50 rxn
EUR 169

Blood Direct PCR Kit V2

PD103-02 200 rxn
EUR 354

Mouse Direct PCR Kit (For Genotyping)

B40013 200 ractions
EUR 209
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B40015 500 ractions
EUR 409
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45012 1ml*5 (500 ractions)
EUR 280
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45013 1ml*20 (2000 ractions)
EUR 865
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

FastAmp Plant Direct PCR Kit - 250 Reactions

4612 1/EA
EUR 164

FastAmp Plant Direct PCR Kit - 1250 Reactions

4615 1/EA
EUR 518

Direct RT-PCR Kit wioRealStart DNA Polymerase

FYT507-100P 100 Preps Ask for price

Direct RT-PCR Kit wioRealStart DNA Polymerase

FYT507-50P 50 Preps Ask for price

Allele-In-One Human Blood Direct PCR Kit

ABP-PP-BD02100 100 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Allele-In-One Human Blood Direct PCR Kit

ABP-PP-BD02500 500 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02100 100 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02500 500 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Direct RT-PCR Kit wi RealStart DNA Polymerase

FYT506-100P 100 Preps Ask for price

Direct RT-PCR Kit wi RealStart DNA Polymerase

FYT506-50P 50 Preps; Ask for price

MULTIPLEX KIT PCR MASTITIS PCR kit

PCR-MPX218-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS

MULTIPLEX KIT PCR MASTITIS PCR kit

PCR-MPX218-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS

MULTIPLEX KIT PCR Babesia & Theileria PCR kit

PCR-MPX401-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria

MULTIPLEX KIT PCR Babesia & Theileria PCR kit

PCR-MPX401-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)

105-540 20 rcs x 50 µl
EUR 164

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)

105-542 100 rcs x 50 µl
EUR 549

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)

105-544 1000 rcs x 50 µl
EUR 4189

Halobacillus PCR kit

PCR-A635-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Halobacillus

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