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PMA (Propidium Monoazide)

Product Description

PMA (propidium monoazide) is a photo-reactive DNA-binding dye used in the viability PCR (V-PCR) of microorganisms such as bacteria, viruses and fungi. PMA is available as a 1 mg lyophilized solid or 20 mM solution in water. Also try PMAxx (40069), a better alternative to PMA.

  • Selectively detect viable cells using qPCR
  • Validated in hundreds of publications
  • Dead cell-specific dye, binds to DNA
  • Covalently attaches to DNA after photoactivation
  • Available as 1 mg lyophilized solid or 20 mM solution in water
  • λAbs = 464 nm (before photolysis); λAbsEm= ~510/~610 nm (with DNA/RNA, after photolysis)

To learn more about the advantages of determining microbial or cell viability using viability PCR, visit the Viability PCR Technology Page.

Product Catalog Number Unit Size Format
PMA Dye 40013 1 mg Lyophilized solid
PMA Dye, 20 mM in H2O 40019 100 uL (20 mM in water) Solution
pma cell dna
pma cell dna

About PMA

PMA dye is a DNA modifier invented by Biotium scientists. It is a photo-reactive dye that binds to DNA with high affinity. Upon photolysis with visible light, the PMA dye covalently binds to DNA. This modified DNA cannot be amplified by PCR. The dye is designed to be cell membrane-impermeable. Thus, in populations of living and dead cells, only dead cells are susceptible to DNA modification due to compromised cell membranes. This unique feature of PMA dye makes it highly useful in the selective detection of live bacteria by qPCR.

Since Biotium first developed the PMA dye, there have been hundreds of publications on the use of the dye in multiple sample types, including dozens of bacterial strains, biofilms, yeasts, fungi, viruses, and eukaryotic cells. It has been used in applications such as food and water safety and environmental testing, and has been used in combination with qPCR, NextGen Sequencing (NGS), Sanger sequencing, and loop-mediated isothermal amplification (LAMP).

PMAxx technology is covered by approved and/or pending US and international patents.

PMA
PMA

Summary

Propidium monoazide (PMA) is a highly selective dye that penetrates only membrane-compromised, dead microbial cells and inhibits both DNA extraction and amplification. PMA has been widely used for discrimination between living and dead microbial cells; However, the application of PMA in phytoplankton studies has been limited. In this study, we attempted to evaluate its applicability for the differentiation of viable phytoplankton. We tested PMA on seven phytoplankton species, Microcystis aeruginosa, Anabaena sp., Aphanizomenon sp., Synechocystis sp., Cryptomonas ovata, Scenedesmus obliquus, and Nitzschia apiculata. The major phytoplankton taxa Cyanobacteria (the first four species, Cryptophyta, and Bacillus chlorophyta), Chlorophyta, and Cryptomonas ovata. respectively as representative. Our results showed that the application of PMA to freshwater living phytoplankton has the potential to distinguish viable from dead cells as in microbial studies. Notably, PMA differentiated viable from dead cells in cyanobacterial species rather than other phytoplankton taxa under our experimental conditions. However, our results also showed that it may be necessary to accommodate different conditions affecting PMA treatment efficiency in order to expand its applicability to other phytoplankton. Although not all factors contributing to the effects of PMA could be evaluated, our study showed the applicability of PMA-based molecular approaches, which can be convenient quantitative methods to distinguish living from dead phytoplankton in freshwater ecosystems. Huh. Determining optimal treatment conditions for other phytoplankton species may enhance the efficacy of PMA-based molecular approaches.

 

 

PMA

40013 1MG
EUR 196
Description: Minimum order quantity: 1 unit of 1MG

pMA-SpCas9-g3

PVT17837 2 ug
EUR 300

pMA-SpCas9-g4

PVT17838 2 ug
EUR 300

pMA-SpCas9-g5

PVT17843 2 ug
EUR 300

pMA-SpCas9-g2

PVT17844 2 ug
EUR 300

pMA-SpCas9-g1

PVT17848 2 ug
EUR 300

pMA-SpCas9-g9

PVT17849 2 ug
EUR 300

pMA-SpCas9-g8

PVT17850 2 ug
EUR 300

pMA-SpCas9-g10

PVT17852 2 ug
EUR 300

pMA-SpCas9-g7

PVT17854 2 ug
EUR 300

pMA-SpCas9-g6

PVT17855 2 ug
EUR 300

PMA (PKC kinase activator)

SIH-508-1MG 1 mg
EUR 126
  • PMA (Phorbol 12-myristate 13-acetate) is a phorbol ester. It is used experimentally as a reversible protein kinase C (PKC) activator. It has been shown to be a potent tumor promoter.
Description: The substance PMA is a pkc kinase activator. It is synthetically produced and has a purity of ?99%. The pure substance is colorless amorphous film or foam which is soluble in DMSO (25 mg/ml), 100% ethanol (25 mg/ml), acetone, ether or dimethyl formamide; almost insoluble in aqueous buffers.

PMA (PKC kinase activator)

SIH-508-5MG 5 mg
EUR 266
  • PMA (Phorbol 12-myristate 13-acetate) is a phorbol ester. It is used experimentally as a reversible protein kinase C (PKC) activator. It has been shown to be a potent tumor promoter.
Description: The substance PMA is a pkc kinase activator. It is synthetically produced and has a purity of ?99%. The pure substance is colorless amorphous film or foam which is soluble in DMSO (25 mg/ml), 100% ethanol (25 mg/ml), acetone, ether or dimethyl formamide; almost insoluble in aqueous buffers.

PMA-Lite LED Photolysis Device

E90002 1EA
EUR 2211
Description: Minimum order quantity: 1 unit of 1EA

Phorbol-12-myristate 13-acetate (PMA)

1544-5
EUR 213

Viability PCR Starter Kit with PMA

31075 1kit
EUR 363
Description: Minimum order quantity: 1 unit of 1kit

PMA Enhancer for Gram Negative Bacteria, 5X Solution

31038 16ml
EUR 132
Description: Minimum order quantity: 1 unit of 16ml

Viability PCR Starter Kit with PMA and Enhancer

31076 1kit
EUR 363
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA)

31033 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Mycobacterium tuberculosis (groEL2)

31034 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (nuc)

31035 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA)

31036 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA)

31050 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly)

31051 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip)

31053 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) PMAxx

31033-X 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx

31050-X 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

 

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