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proteinase k

Proteinase K, recombinant

 

Proteinase Ok – one of the generally used enzymes in molecular biology.

Excellent alternative for protein digestion in varied functions.

  • BLIRT – professional in Proteinase Ok manufacturing for over 15 years.
  • Licensed manufacturing course of ISO 13485 ensuing within the highest high quality product with a really low batch-to-batch variability.
  • Massive portions, the opportunity of white labeling, and portioning service – liquid kind within the quantity on the buyer’s request (as much as a number of million samples month-to-month).

 

Thermo Scientific Proteinase Ok is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even within the presence of detergents. Proteinase Ok cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Ok is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Proteinase Ok (Lyophilized)

For Basic Digestion of Protein in Organic Samples

  • Lively over a pH vary of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
  • Purified to take away RNase and DNase actions

Secure at Room Temperature and Simple to Use

  • Lively over pH vary 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% exercise at temperatures as much as 60°C
  • No resuspension or thawing earlier than use
  • Cat.# MC5005, MC5008 provided at a focus of 20mg/ml



Highlights

• Prepared-to-use answer
• Lively in a variety of response circumstances

Functions

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

Be aware

• The really useful working focus of Proteinase Ok is 0.05 to 1 mg/mL. The exercise of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Ok towards autolysis, will increase the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Ok
• Secure over a large pH vary: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum exercise at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.

Proteinase K, recombinant
Proteinase K, recombinant

Proteinase Ok Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular exercise.

Recombinant Proteinase Ok enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the very best high quality product.

Proteinase Ok Molecular Biology Grade (PCR Grade) is lively underneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, throughout nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.

Options

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Excessive exercise and distinctive purity.
  • Lively at excessive temperatures (as much as 56 °C) and denaturing circumstances (e.g. within the presence of urea and/or SDS), which makes it very best for digesting proteins in a wide range of functions.
  • Secure over a large pH vary: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Functions:

  • Extraction of DNA and RNA from totally different beginning supplies.
  • Purification of goal materials from contaminating proteins.
  • Elimination of DNases and RNases throughout nucleic acids isolation.

 

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Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

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