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Bioassay

QuantiChrom Urea Assay Kit

Utility

  • For quantitative dedication of urea and analysis of drug results on urea metabolism.

Key Options

  • Delicate and correct. Use 5 μL samples. Linear detection vary 0.08 mg/dL (13 μM) to 100 mg/dL (17 mM) urea in 96-well plate assay.
  • Easy and high-throughput. The process includes addition of a single working reagent and incubation for 20 min. Could be readily automated as a high-throughput assay for 1000’s of samples per day.
  • Improved reagent stability and flexibility. The optimized formulation has enormously enhanced reagent and sign stability. Cuvet or 96-well plate assay.
  • Low interference in organic samples. No pretreatments are wanted. Assays could be straight carried out on uncooked organic samples i.e., within the presence of lipid and protein.

Technique

  •  OD520nm (Chemical)

Samples

  •  Serum, plasma, urine, milk, cell/tissue tradition, bronchoalveolar lavage (BAL), meals, beverage and atmosphere

Species

  •  All

Measurement

  •  100 assessments

Detection Restrict

  •  0.08 mg/dL (13 μM)

Shelf Life

  •  12 months

Extra Particulars

urea-assay-kit
urea-assay-kit

 

  •  Urea is primarily produced within the liver and secreted by the kidneys. Urea is the main finish product of protein catabolism in animals. It’s the major automobile for removing of poisonous ammonia from the physique. Urea dedication could be very helpful for the medical clinician to evaluate kidney operate of sufferers. Basically, elevated urea ranges are related to nephritis, renal ischemia, urinary tract obstruction, and sure extrarenal illnesses, e.g., congestive coronary heart failure, liver illnesses and diabetes. Decreased ranges point out acute hepatic insufficiency or could outcome from over-vigorous parenteral fluid remedy.
  • Easy, direct and automation-ready procedures for measuring urea focus or blood urea nitrogen BUN in organic samples have gotten standard in Analysis and Drug Discovery. BioAssay Techniques urea assay equipment is designed to measure urea straight in organic samples with none pretreatment. The improved Jung technique makes use of a chromogenic reagent that types a coloured advanced particularly with urea. The depth of the colour, measured at 520nm, is straight proportional to the urea focus within the pattern. The optimized formulation considerably reduces interference by substances within the uncooked samples.

For quantitative dedication of urea and analysis of drug results on urea metabolism.

 

  • Delicate and correct, use 5 μl samples
  • Linear detection vary 0.08 mg/dl (13 μM) to 100 mg/dl (17 mM) urea in 96-well plate assay
  • Easy and high-throughput
  • The process includes addition of a single working reagent and incubation for 20 min
  • Could be readily automated as a high-throughput assay for 1000’s of samples per day
  • Technique: OD520 nm (Chemical)
  • Samples: Serum, plasma, urine, milk, cell/tissue tradition, bronchoalveolar lavage (BAL), meals, beverage and atmosphere

Improved reagent stability and flexibility. The optimized formulation has enormously enhanced reagent and sign stability. Low interference in organic samples. No pretreatments are wanted. Assays could be straight carried out on uncooked organic samples i.e., within the presence of lipid and protein. Cuvet or 96-well plate assay. Urea is primarily produced within the liver and secreted by the kidneys. Urea is the main finish product of protein catabolism in animals.

It’s the major automobile for removing of poisonous ammonia from the physique. Urea dedication could be very helpful for the medical clinician to evaluate kidney operate of sufferers. Basically, elevated urea ranges are related to nephritis, renal ischemia, urinary tract obstruction, and sure extrarenal illnesses, e.g., congestive coronary heart failure, liver illnesses and diabetes.

Decreased ranges point out acute hepatic insufficiency or could outcome from over-vigorous parenteral fluid remedy. Easy, direct and automation-ready procedures for measuring urea focus or blood urea nitrogen BUN in organic samples have gotten standard in Analysis and Drug Discovery. BioAssay Techniques urea assay equipment is designed to measure urea straight in organic samples with none pretreatment.

The improved Jung technique makes use of a chromogenic reagent that types a coloured advanced particularly with urea. The depth of the colour, measured at 520 nm, is straight proportional to the urea focus within the pattern. The optimized formulation considerably reduces interference by substances within the uncooked samples.

 

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  • Product category: Electrophoresis Related/Reagents
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