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recombitant sars cov 2 ProteoSilver Silver Stain Kit

FD Rapid GolgiStain Kit (large)

Product Name Catalog # Size Downloads Technical Assistance
FD Rapid GolgiStain™ Kit (large) PK401 For up to 50 mouse brains Manual
References
MSDS
Video
Protocol/Paper

Golgi–Cox impregnation has been one of the most effective techniques to study the normal and abnormal morphology of neurons 1, 2 as well as glia. Using the Golgi technique, subtle morphological changes in neuronal dendrites and dendritic spines have been discovered in the brains of animals treated with drugs as well as in post-mortem brains of patients with neurological diseases 3, 4 . However, the unreliability and time-consuming process of Golgi staining have been major obstacles to the widespread application of this technique.

The FD Rapid Golgistein Kit is designed based on the principle of the methods described by Ramón- Moliner 2, Glaser, and Van der Loos 5. This kit has not only dramatically improved and simplified the Golgi-Cox technique, but also proved to be extremely reliable and sensitive for displaying morphological details of neurons and glia, particularly dendritic spines. The FD Rapid GolgiStain Kit has been extensively tested and widely used on brains from several species of animals as well as postmortem human brain samples.

Kit contents:
Store at room temperature

Solution A                                         250 ml
Solution B                                         250 ml
Solution C                                         250 ml x 2
Solution D                                         250 ml
Solution E                                         250 ml
Glass Specimen Retriever              2
Natural hair paintbrush                 2
Dropping bottle                                1

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Materials required but not included:

  • Double distilled or deionized water.
  • Plastic or glass tubes or vials.
  • Histological supplies and equipment, including gelatin-coated microscope slides, coverslips, staining jars, ethanol,  xylene or xylene substitutes, resinous mounting medium (e.g. Permount®), and a light microscope.

General description

Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver is an ideal product for any protein scientist. The kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver leads to superior detection of low abundance proteins.

Application

ProteoSilver Silver Stain Kit has been used for staining of the gels for visualizing proteins.

Features and Benefits

  • Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
  • Optimized protocols help establish conditions for best results
  • High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
  • MALDI Compatible – Protein spots of interest can be further characterized by mass spectrometry
  • Room Temperature Stability allows easy and convenient storage

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC.

 

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