Most popular and consistent embedding medium for cryotomy
Tissue-Tek OCT compound is used to quickly embed fresh tissue samples into frozen sections using a cryostat. Tissue-Tek OCT Compound is a formulation of clear, water-soluble glycols and resins to encapsulate tissue samples and bond to a solid for continuous sectioning at cryostat working temperatures of -10 °C and below. Provides metrics.
Unwanted background blurring is eliminated because there is no residue left on the slide during staining.
OCT stands for optimum cutting temperature. Tissue-Tek OCT compound’s high viscosity results in rapid freezing for optimum segment quality.
OCT compound is sold in 4 oz (118 mL) squeeze bottles; 12/case.
tissue-tek-paraform
What exactly is in the TissueTech OCT compound and what effect does it have on the enzyme activity of tissues?
Hi all, I’m working with cryo skin samples and what I’m trying to do in situ zymography (imagine enzyme activation with substrates that become fluorescent). Skin samples are prepared with TissueTek, then snap-freezed in liquid nitrogen and then cut using a cryostat. However, I can’t find what is actually in TissueTek, as this may be the reason for the significant effect on enzyme activity (pH?). Does anyone know if embedding a tissue with TissueTec can affect enzyme activity in the tissue?
Specs for this merchandise
Model Identify
EMS
Merchandise Weight
113 grams
Mannequin Quantity
62550-01
Variety of Gadgets
1
Half Quantity
62550-01
tissue-tek
Product particulars
Is Discontinued By Producer : No
Package deal Dimensions : 19.three x 5.three x four cm; 113.four Grams
Date First Accessible : 13 January 2020
Producer : Electron Microscopy Sciences
ASIN : B00Ok33LEBS
Merchandise mannequin quantity : 62550-01
Producer : Electron Microscopy Sciences
Merchandise Weight : 113 g
Web Amount : 1 Answer
The Tissue-Tek optimum chopping temperature (O.C.T.) resolution consists of water-soluble glycols and resins that give a specimen matrix for cryostat sectioning at temperatures of –10°C (14°F) and beneath. This product doesn’t go away any residue on slides throughout and after staining course of. It’s packaged and bought individually in a 118mL (4oz.) bottle.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Ornithine Carbamoyl Transferase (OCT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Ornithine Carbamoyl Transferase (OCT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Human Ornithine Carbamoyl Transferase (OCT) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Ornithine Carbamoyl Transferase (OCT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Human Ornithine Carbamoyl Transferase (OCT) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Ornithine Carbamoyl Transferase (OCT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Rat Ornithine Carbamoyl Transferase (OCT) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ornithine Carbamoyl Transferase (OCT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Rat Ornithine Carbamoyl Transferase (OCT) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ornithine Carbamoyl Transferase (OCT) in samples from serum, plasma, tissue homogenates or other biological fluids.
