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RNA Purifications kit

Total RNA Purification Kits

The purification methods of our Total RNA Purification Kit range all the way from organic extraction reagents to spin columns and magnetic beads.

Starting with high quality, pure, and intact total RNA is critical for many experiments (such as RT-PCR, qRT-PCR, array analysis, northern blot, nuclease protection assay, and RNA-sequencing). The table below will help you choose the right Invitrogen product to purify total RNA from your specific sample type and sample size.

The Thermo Scientific GeneJet RNA Purification Kit is a simple and efficient system for the purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria and yeast.

The kit uses silica-based membrane technology in the form of a convenient spin column, which eliminates the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extraction.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJet RNA Purification Kit in 15 min after the lysis step. High-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlight

• Universal – can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell culture, mammalian tissue, insects, yeast and bacteria
• Efficient – high yield of total RNA.
• Fast – 15 min after the lysis step
• Pure – an A260 / 280 ratio > 1.9
• Convenient – Spin columns are capped and assembled with collection tubes

 

RNA purification
RNA purification

Application

• High purity suitable for all conventional molecular biology processes, fast extraction of RNA including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease Protection Assay in Proteinase K Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, Nuclease-free GeneJET RNA Purification Column (Pre-assembled with Collection Tubes) Collection Tube (2 ml) Collection Tube (1.5 ml) ) Are included. Etiquette

General Details

Sigma’s GenElute Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, plasma, serum, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). RNA is preferentially purified from other cellular components, such as proteins, without the use of phenol or chloroform. Purified RNA is of the highest integrity, and can be used in many downstream applications, including real-time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays.

Isolate true total RNA, including siRNA and microRNA
Rapidly remove contaminating genomic DNA without the use of enzymes
No phenol or chloroform extraction
Purify high quality RNA in 20 min
Extract RNA as small as a single cell
isolate from different types of samples
Application

RNA purification kit
RNA purification kit

The GenElute Total RNA Purification Kit is used for the extraction of total RNA:

Detection of hepatitis C virus from whole blood samples using the reverse transcriptase-polymerase spiral reaction (RT-PSR) system.
To determine the effects of curcumin and silymarin on virulence and carbapenemase genes among multidrug-resistant Escherichia coli clinical isolates from overnight culture of bacterial strains in TSB medium.
The GenElute™ Total RNA Purification Kit is used to:

Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
RT-PCR for both large mRNA and small RNA including miRNA
expression outline
Next generation sequencing for RNA and miRNA
Discovery of miRNA from Plasma
MicroRNA cloning and amplification
PCR-based virus detection
PCR-based detection of viable bacteria
northern blotting
RNase protection
Primer Extension
miRNA array
RNA Seq
nanostring
fluidigm
droplet/digital PCR
Isolate total RNA from rat kidney tissue

 

RNA Purification Kit

abx098095-25rxns 25 rxns
EUR 230
  • Shipped within 5-10 working days.

RNA Purification Kit

MB1006-250preps 250 preps
EUR 397

RNA Purification Kit

MB1006-50preps 50 preps
EUR 166

PureXtract RNA Purification Kit

R6111-050 50 prep
EUR 280

PureXtract RNA Purification Kit

R6111-200 200 prep
EUR 874

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL 96 assays
EUR 572

RNA isolater Total RNA Extraction Reagent

R401-01 100 ml
EUR 133

Green RNA Lysis Kit 50

GREENE1-RNA 50pack
EUR 180
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250

GREENE5-RNA 250pack
EUR 571
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Navy RNA Lysis Kit 50

NAVYE1-RNA 50pack
EUR 198
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250

NAVYE5-RNA 250pack
EUR 651
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Pink RNA Lysis Kit 50

PINKE1-RNA 50pack
EUR 178
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250

PINKE5-RNA 250pack
EUR 561
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50

REDE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250

REDE5-RNA 250pack
EUR 622
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50

YELLOWE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250

YELLOWE5-RNA 250pack
EUR 624
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

AfterTri-RNA Purification Mini Kit (200prep)

FAATR-001-1 200 preps
EUR 259

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 402

Total RNA Extraction Kit

K2014005 1 kit
EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA Isolation Kit

P-9100 50 samples
EUR 347.25
Description: The best epigenetics products

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